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Technologies

Immunology & Infectiology

IDMIT has a long experience both in the immuno-monitoring of the immune system molecular responses, and in the study/evolution of viremia in the frame of drug, therapeutic or vaccine assays.

 

technologies-bioassays

 

These technical skills are crucial to:

  • to understand and model mechanismes of host immune responses to vaccines, drugs and therapeutics,
  • to follow the infectious pathogen titers and localizations (viral reservoirs and pharmacologic sanctuaries),
  • to assess immunogenicity of new therapeutic (peptides, proteins) and vaccine strategies,
  • to progress in vaccine and innovative therapeutics efficacy and safety evaluation in experimental models of infection.

 

ELISA, ELISpots, Luminex, PCR, real-time PCR, ICS, in situ hybridization, serology, immuno-histology, immuno-phenoptyping are regularly performed. In addition, RNAsequencing and functional genomics (Next generation Sequencing: NGS) is done in collaboration with the Genome Institute (CEA-Evry, France).

 

Applied in fondamental, preclinical or clinical (cohorts of human biological samples) research programs, on  blood, plasma, serum, body secretions (broncoalveolar secretions…), fluids (salivary, seminal fluid…), tissue homogenates,  IDMITs’ Bio-assays activities allow:

  • innate immune responses
  • antibody measurements (IgM, IgG…)
  • serum neutralization activity follow-up
  • cell-mediated specific responses (CD4+, CD8+, dendritic cells…)/multiplex dosages:

– cytokines profiling (longitudinal monitoring of inflamatory markers)

– expression profiles of interferon subtypes

  • pre, post-immunization, treatment, immune analyses
  • immune-cells phenotypes
  • pathogen (virological, bacterial, parasites) parameters:

                – determination of pathogen proteins

               – plasma pathogen RNA or DNA levels

               – pathogen (viruses…) titers in tissue homogenates.

 

  • Generated data are differently managed:
  • centralized storage,
  • long-term traceability and safety
  • multi-users and projects format
  • electronic lab notebook
  • real-time analysis
  • meta-analysis:

               – inter-project reports

               – combined analysis of different types of data

  • modelization assays via our Bioinformtic group.